Method
1. Place 200 mL of the 10 percent washed red blood cells into a microfuge tube.
2. Centrifuge for 15 seconds.
3. Remove the supernatant.
4. Shake the serum sample and remove 500 mL of serum. Some samples may contain less volume. Use a new tip for each sample.
5. Add the serum to the red blood cells in the microfuge tube. Mix gently.
6. Stand for not less than 30 minutes at 4°C.
7. Centrifuge for 15 seconds.
8. Remove the serum (supernatant) immediately and transfer to a clean microfuge tube.
9. Store the adsorbed sera at 4°C overnight or at -20°C for longer periods.