Biological control,
including the treatment of crop residues with antagonists, in order to reduce pathogen inoculum of
FHB, holds considerable promise. Ten Trichoderma isolates, previously selected for their ability to
grow in the presence of DON, were preliminarily investigated as potential antagonists against
Fusarium culmorum and F. graminearum mycotoxigenic strains in plate confrontation assays
The
three Trichoderma isolates showing antibiosis and mycoparasitism were evaluated for their
capacity to inhibit DON production by F. graminearum and F. culmorum on two natural
substrates. The expression of some chitinase-encoding genes by the two best resulting
Trichoderma strains, during interaction with F. culmorum and F. graminearum, was monitored. All
investigated genes from chitinase subgroups A, B and the new subgroup C responded to
mycoparasitic conditions and were upregulated before contact and/or when in contact with the
host. T. gamsii 6085, the best antagonist, was finally used in a competition test against F.
culmorum and F. graminearum on natural substrates, using a qPCR approach to evaluate its
effect on the pathogen’s growth and DON production in haulms and rice. This test confirmed the
ability of T. gamsii 6085 to antagonize the pathogens on rice. On wheat haulms, an extreme
oligotrophic environment, T. gamsii 6085 seemed to develop very poorly and the growth of both
the pathogens was unaffected by the presence of the antagonis
Biological control,including the treatment of crop residues with antagonists, in order to reduce pathogen inoculum ofFHB, holds considerable promise. Ten Trichoderma isolates, previously selected for their ability togrow in the presence of DON, were preliminarily investigated as potential antagonists againstFusarium culmorum and F. graminearum mycotoxigenic strains in plate confrontation assaysThethree Trichoderma isolates showing antibiosis and mycoparasitism were evaluated for theircapacity to inhibit DON production by F. graminearum and F. culmorum on two naturalsubstrates. The expression of some chitinase-encoding genes by the two best resultingTrichoderma strains, during interaction with F. culmorum and F. graminearum, was monitored. Allinvestigated genes from chitinase subgroups A, B and the new subgroup C responded tomycoparasitic conditions and were upregulated before contact and/or when in contact with thehost. T. gamsii 6085, the best antagonist, was finally used in a competition test against F.culmorum and F. graminearum on natural substrates, using a qPCR approach to evaluate itseffect on the pathogen’s growth and DON production in haulms and rice. This test confirmed theability of T. gamsii 6085 to antagonize the pathogens on rice. On wheat haulms, an extremeoligotrophic environment, T. gamsii 6085 seemed to develop very poorly and the growth of boththe pathogens was unaffected by the presence of the antagonis
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