The extracts were filtered through Whatman No. 1 filter paper, centrifuged at 5000 × g at room temperature for 10min and the supernatant obtained was used as the acid protease.
The extractswere filtered through Whatman No. 1 filter paper, centrifugedat 5000 × g at room temperature for 10min and the supernatantobtained was used as the acid protease.