This study was conducted to determine the effects of supplementation of dried peppermint(Mentha piperita L.)leaves in laying hen diets on laying performance, egg quality, and serummetabolic profile. A total of one hundred and fifty Hy-Line Brown laying hens (64-weekold),were assigned to five treatment diets including dry peppermint leaves at 0, 5, 10, 15,or 20 g/kg, respectively, for 12 weeks. Each treatment had six replicates with five hens each.Over the course of the trial, incremental dietary peppermint leaves significantly increased(linear, P<0.001) egg weight, egg production, egg mass and feed intake from 64–68, 68–72,72–76 and 64–76 weeks of age. Moreover, feed conversion ratio was linearly decreased(P<0.001) with increasing levels of peppermint in laying hens diet. The inclusion of 20 g/kgpeppermint resulted in overall best performance. Eggshell percentage, eggshell thicknessand Haugh unit of hens fed diets supplemented with peppermint leaves were greater(P<0.01) than that of hens fed the control diet. However, peppermint supplementationdid not influence other egg quality characteristics like albumen and yolk percentages andalbumen height. Notably, serum cholesterol significantly decreased (P<0.001) but serumtotal proteins increased (P=0.015) with the increasing peppermint leaves levels. It can beconcluded that peppermint leaves can be used as an effective feed additive to improveperformance of laying hens during the late laying period
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