Chitosan samples dried in the oven
at 90 1C were solubilized in aceticacid and submitted to titration
with 0.1 M NaOH , yield in gacurve with two in flection points.
The difference in NaOH volume used between the two in flection
points corresponds to the acidc on sumed for the salification of the
amino groups of chitosan, and the degree of acetylation was
calculated by