Percoll gradient: 1mL of thawed sample was placed in a 15-mL conical centrifugation tube above a column com- posed of 2mL of 45% Percoll (Biochrom AG, Berlin, Germany) over 2mL of 90% Percoll.
Percoll gradient: 1mL of thawed sample was placed in a15-mL conical centrifugation tube above a column com-posed of 2mL of 45% Percoll (Biochrom AG, Berlin,Germany) over 2mL of 90% Percoll.