As shown in Fig. 4G, germacrone treatment dramatically promoted
the translocation of cytochome c from the mitochondria (Fig. 4G Cyto a)
to cytosol (Fig. 4G Cyto b). Germacrone significantly activated caspases
3, 7, and 9, as demonstrated by the appearance of the cleaved fragments
at 200 μM. Furthermore, germacrone also triggered PARP cleavage as
indicated by the appearance of PARP fragments (Fig. 4G).The full length
of PARP was dose-dependently increased after germacrone treatment.