Data were subjected to one-way analysis of variance (ANOVA)
usingSPSS forWindows (SPSS®, version 10.0, Chicago, USA). Where
there was statistical significance (p ≤ 0.05), the mean values were
furtherseparated using the Duncan’s multiple range test. The levels
ofsignificant difference between the mean values ofthe tissue cultured and acclimatized plantlets were determined using the t-test
(SigmaPlot Version 8.0).