Aqueous extraction experiments were conducted under the optimum operating conditions determined from the experiments described above, for bran treated by each method after 0, 10, 20, 30, 40 and 50 days. Rice bran (200 g) was mixed with 2 l of water at 60 ◦C, pH 12.0 for 30 min at 800 rpm. RBO was obtained as per the method described above. The amount of oil obtained were weighed and recorded.