2.8. Antioxidant activity
2.8.1. Preparation of extract
Antioxidant was extracted according to Xu et al. (2008) with slight modifications. Equal parts of mango juice were added to 80% methanol to purify the sample. The mixture was placed in a shaking incubator (Shellab Orbital Shaking Incubator S14,OR, USA) at 250 rpm for 30 min at room temperature, and then centrifuged. The supernatant was used for the analysis of antioxidant activity.