We selected the recombinant FragA a-galactosidase because it has a slightly basic isoelectric point similar to the E. meningosepticum enzyme; using the same glycine buffer system as for group A RBCs, we effectively produced, by enzymatic conversion of blood group B RBCs with the FragA enzyme, B-ECO RBCs that type as group O by licensed reagents and methods used in clinical blood bank practice (Table 3)