2.3. Genotyping of Giardia duodenalis
DNA was extracted directly from ELISA-positive faecal
samples with the extraction kit QIAamp DNA Stool Mini
Kit (QIAGEN Gmbh, Hilden, Germany). The amplification
of the 432 bp region of the glutamate dehydrogenase gene
(gdh) and the genotyping of G. duodenalis were performed
according to the PCR-RFLP protocol published by Read et al.
(2004).