Dark fermentative hydrogen production
Dark fermentative hydrogen production was performed at 37 °C in a 3 L fermentor with working volume of 500 mL using food waste hydrolysate. The inoculum size of bacteria (Biohydrogenbacterium R3) was 2% (v/v). Dark fermentation was conducted with external N2 sparging into the broth at a rate of 0.5 vvm and agitated at 300 rpm by magnetic stirrer. Fermentation pH was automatically controlled between 4.0 and 4.6 using 5 M NaHCO3 and 0.005 M H2SO4. The fermentative hydrogen production from food waste hydrolysate was carried out in duplicate.