Ethanolic extracts prepared from the stems of T. crispa are commonly used to treat fever, diabetes and jaundice in traditional medicine in Malaysia, Thailand and Philippines. The results of this study demonstrate the hepatotoxicity of TAA is potentiated by the plant extract when administered daily for 8 weeks. This study also emphasizes the usefulness of a dual strategy involving in vitro and in vivo experiments on medicinal plants commonly used in traditional medicine, whose safety has not been yet been established.