These microorganisms were selected based on
the solubilization efficiency of inorganic and organic phosphate sources in a modified Pikovskaya’s liquid
medium culture containing sodium phytate (phytic acid), soybean lecithin, aluminum phosphate (AlPO4),
and tricalcium phosphate (Ca3(PO4)2). The isolates were identified based on nucleotide sequence data
from the 16S ribosomal DNA (rDNA) for bacteria and actinobacteria and internal transcribed spacer (ITS)
rDNA for fungi. Bacteria produced the greatest solubilization in medium containing tricalcium phosphate.
Strains B17 and B5, identified as Bacillus sp. and Burkholderia sp., respectively, were the most
effective, mobilizing 67% and 58.5% of the total P (Ca3(PO4)2) after 10 days, and were isolated from the
rhizosphere of the P efficient L3 maize genotype, under P stress. The fungal population was the most
effective in solubilizing P sources of aluminum, phytate, and lecithin. A greater diversity of P-solubilizing
microorganisms was observed in the rhizosphere of the P efficient maize genotypes suggesting that the P
efficiency in these cultivars may be related to the potential to enhance microbial interactions of P-solubilizing
microorganisms.
These microorganisms were selected based onthe solubilization efficiency of inorganic and organic phosphate sources in a modified Pikovskaya’s liquidmedium culture containing sodium phytate (phytic acid), soybean lecithin, aluminum phosphate (AlPO4),and tricalcium phosphate (Ca3(PO4)2). The isolates were identified based on nucleotide sequence datafrom the 16S ribosomal DNA (rDNA) for bacteria and actinobacteria and internal transcribed spacer (ITS)rDNA for fungi. Bacteria produced the greatest solubilization in medium containing tricalcium phosphate.Strains B17 and B5, identified as Bacillus sp. and Burkholderia sp., respectively, were the mosteffective, mobilizing 67% and 58.5% of the total P (Ca3(PO4)2) after 10 days, and were isolated from therhizosphere of the P efficient L3 maize genotype, under P stress. The fungal population was the mosteffective in solubilizing P sources of aluminum, phytate, and lecithin. A greater diversity of P-solubilizingmicroorganisms was observed in the rhizosphere of the P efficient maize genotypes suggesting that the Pefficiency in these cultivars may be related to the potential to enhance microbial interactions of P-solubilizingmicroorganisms.
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