The
Petri dishes were incubated at room temperature
(30+2°C) for 5 days and the creamy white colonies
of the yeast were transferred to fresh PDA plates.
The most effective yeast isolate was screened by
a dual culture test. Pure culture of the selected
yeast isolate was grown on PDA and stored at 5°C
until use. Identification of this yeast isolate was
carried out by BIOTEC, the National Science and
Technology Development Agency and the result
revealed it as Issatchenkia orientalis.