A. Constant temperature:
Cultured at constant temperatures 15, 20, 25 and 30 8C
for 2 weeks.
B. Changing to high temperature:
a. Slow increase
1. Cultured at 20 8C for 1 day, temperature increased to
25 8C at 1 8C/day for 5 days, held at 25 8C for 8 days.
2. Cultured at 20 8C for 1 day and increase the
temperature to 30 8C at a speed of 1 8C/day for 10
days, then stayed at 30 8C for 3 days.
b. Rapid increase
1. Cultured at 20 8C for 7 days and changed the
temperature to 25 8C for 7 days.