The residue was recovered by centrifugation and dried.
The resulting seaweed (25.6 g) then was split into 30 parts for the following process.
0.85 g A. nodosum was suspended in 15 ml 0.1 M HCl solution and put in a 35 ml microwave tube.
Then the microwave tube was placed into the CEM Discover reactor for microwave irradiation at 90 C for 15 min.
After irradiation, the suspension was centrifuged to separate the residual alga, which was washed prior to alginate extraction.
At this stage, the supernatant contains predominately fucoidan, but also traces of alginate.
Therefore, 2% (w/v) CaCl2 solution was added to the liquid fraction and the mixture was maintained overnight at 4 C for alginate removal.
The solid fraction obtained by treatment with CaCl2 was separated by centrifugation.
Double volume of absolute ethanol was added to the resultant filtrate and the ethanol-precipitated fucoidan was recovered by centrifugation, then washed and dried at 40 C.
The supernatant after fucoidan precipitation was concentrated to 5 ml and neutralized for HPAEC analysis
The residue was recovered by centrifugation and dried. The resulting seaweed (25.6 g) then was split into 30 parts for the following process. 0.85 g A. nodosum was suspended in 15 ml 0.1 M HCl solution and put in a 35 ml microwave tube. Then the microwave tube was placed into the CEM Discover reactor for microwave irradiation at 90 C for 15 min.After irradiation, the suspension was centrifuged to separate the residual alga, which was washed prior to alginate extraction. At this stage, the supernatant contains predominately fucoidan, but also traces of alginate.Therefore, 2% (w/v) CaCl2 solution was added to the liquid fraction and the mixture was maintained overnight at 4 C for alginate removal.The solid fraction obtained by treatment with CaCl2 was separated by centrifugation.Double volume of absolute ethanol was added to the resultant filtrate and the ethanol-precipitated fucoidan was recovered by centrifugation, then washed and dried at 40 C. The supernatant after fucoidan precipitation was concentrated to 5 ml and neutralized for HPAEC analysis
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