Materials and methods
Trichoderma asperellum bioproducts
T. asperellum isolates CB-Pin-01 and 01-52 (¼ 03/7-I34) (Molecular
taxonomic based; Unartngam, personal communication)
used in this study were provided by Associate Professor Dr. Chiradej
Chamswarng, Plant Disease Biocontrol Laboratory, Department
of Plant Pathology, Faculty of Agriculture at Kamphaeng
Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon
Pathom province, Thailand. These two isolates were formerly
identified as T. harzianum based on fungal growth andmorphology
(Chamswarng and Intanoo, 2007; Inwang and Chamswarng, 1986).
T. asperellum CB-Pin-01 was cultured on semi-cooked broken
milled rice as a fresh culture bioproduct according to the procedure
developed by Chamswarng and Intanoo (2002). T. asperellum
01-52 was prepared as a wettable pellet bioproduct (1 108 colony
forming units/g; unpublished data).