3.4. Effects of inoculums size on immobilized cells
Inoculums size also played an important role on pectinase production. Higher or lower inoculum size than the optimized con-centration resulted in reduced enzyme production. Fig. 4 shows the pectinase production was produced maximally in 6 nylon scour-ing pad cubes containing 1 × 107spores/ml of inoculums size with9.41 U/mL and 8.07 g/L of fungal growth. Higher or lower num-ber of inoculums sizes produced low enzyme activity. There wereno remarkable different (Duncan, p > 0.05) of pectinase production from size of inoculums 1 × 105and 1 × 106. The maximal fungal growth was achieved when an inoculum of 1 × 107spores/ml wasused and minimal fungal growth was found from inoculums withthe size of 1 × 104spores/ml.