2.6. Statistical analysis methods
Statistical comparisons of mean glucan to ethanol yield by the
IMB strains and S. cerevisiae D5A were using the repeated measurements
analysis and Fisher’s protected least significance difference
(LSD) method for comparing multiple treatments for all sample
times at 24 h and afterward using SAS Release 9.1 software (SAS,
Cary, NC). A repeated measurements analysis and Fisher’s protected
LSD method was also used to compare mean glucan to ethanol
yields for the varied enzyme loading studies with IMB 3 and S.
cerevisiae D5A as well as the mean ethanol yields from the initialbuffer pH experiments with S. cerevisiae D5A. Experiments were
performed in triplicate unless otherwise noted and all statistical
comparisons were made at a 95% confidence interval