After about one to two weeks, depending on how much light the columns receive, some green coloring should appear in the columns receiving light on the illuminated sides. This is mostly due to cyanobacteria and algae, which need light. The column in the dark should remain dark brown. In the column that had egg yolk you may have seen areas of darker green, purple, and/or black coloring develop over time near the bottom—these colorings could be groups of certain anaerobic bacteria: green sulfur bacteria, purple sulfur bacteria and sulfate-reducing bacteria, respectively. Sulfate-reducing bacteria actually eat sulfur and make hydrogen sulfide gas, which is eaten by the green and purple sulfur bacteria. In the column that had newspaper you may have seen some areas of brown, orange, red or purple near the middle—these colorings could be groups of purple nonsulfur bacteria, which need a carbon source to thrive. You may have seen worms, snails, shrimp or other small organisms in the water, but probably not many (if any) in the bottle with the egg yolk, because hydrogen sulfide is toxic to most organisms!
After about one to two weeks, depending on how much light the columns receive, some green coloring should appear in the columns receiving light on the illuminated sides. This is mostly due to cyanobacteria and algae, which need light. The column in the dark should remain dark brown. In the column that had egg yolk you may have seen areas of darker green, purple, and/or black coloring develop over time near the bottom—these colorings could be groups of certain anaerobic bacteria: green sulfur bacteria, purple sulfur bacteria and sulfate-reducing bacteria, respectively. Sulfate-reducing bacteria actually eat sulfur and make hydrogen sulfide gas, which is eaten by the green and purple sulfur bacteria. In the column that had newspaper you may have seen some areas of brown, orange, red or purple near the middle—these colorings could be groups of purple nonsulfur bacteria, which need a carbon source to thrive. You may have seen worms, snails, shrimp or other small organisms in the water, but probably not many (if any) in the bottle with the egg yolk, because hydrogen sulfide is toxic to most organisms!
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