Three treatment protocols were used :the venom was preincubated at
room temperature with the crude extract(100mg/kg)or arjunolic
acid (30mg/kg)for 30min before the i.m.injection;the extract
(100mg/kg) or arjunolic acid(30mg/kg)was orally administered
15min before the venom injection;and the post-treatment,where
the animals received the extract(100mg/kg)or arjunolic acid
(30 mg/kg) i.p. injection 5min after the venom injection.The
doses used here were based on the dose-response study(not
shown),fromwherewechosethemosteffectiveones.Thethigh
areawasmeasuredusingacaliperrule.