Our results demonstrated that in the absence of metabolic activation, KalmColdTM did not exhibit any mutagenicity towards
S. typhimurium strains TA98 and TAMix up to a concentration of 5000 lg/ml.
This implies that KalmColdTM neither produced frame shift mutations in TA98 nor base pair mutations in TAMix strains
of S. typhimurium.
The addition of KalmColdTM at concentrations ranging from 16 lg/ml to 5000 lg/ml did not lead to a reproducible increase in the number of revertant colonies at one or more concentrations.
The fold inductions in revertant histidine colonies over the solvent control were found to be less that 2.0 in both the mutant strains.