Table 1. The effect of different concentrations of BAP and NAA on shoot regeneration and growth from leaf cultures of Sinningia
speciosa after 6 weeks in culture.
Hormones
(mg/L)
Number of shoots/explant
Shoot length
(cm)
BAP 1 3.5 ± 0.4 0.72 ± 0.2
BAP 2 5.2 ± 0.4 0.84 ± 0.1
BAP 4 6.7 ± 0.6 0.95 ± 0.1
BAP 1 + NAA 0.1 7.8 ± 0.9 0.82 ± 0.1
BAP 2 + NAA 0.1 12.3 ± 0.8 1.19 ± 0.1
BAP 4 + NAA 0.1 11.3 ± 1.3 1.10 ± 0.1
Each value is the mean ± standard error of 3 repeated experiments, with 50 explants used in each treatment.
Table 2. The effect of different concentrations of AgNO3 on shoot regeneration and growth from leaf cultures of Sinningia speciosa
after 6 weeks in culture on regeneration medium (MS medium with 2.0 mg/L BA and 0.1 mg/L NAA).
AgNO3
(mg/L)
Number of shoots/explant
Shoot growth
(cm)
0 12.3 ± 0.8 12.1 ± 0.1
1 13.7 ± 1.2 13.1 ± 0.1
3 19.7 ± 1.1 1.5 ± 0.2
7 23.9 ± 1.6 1.7 ± 0.2
10 16.2 ± 1.3 1.3 ± 0.2
20 13.8 ± 1.2 0.9 ± 0.1
Each value is the mean ± standard error of 3 repeated experiments, with 50 explants used in each treatment.
Table 3. The effect of different concentrations of putrescine on shoot regeneration and growth from leaf cultures of Sinningia
speciosa after 6 weeks in culture on regeneration medium (MS medium with 2.0 mg/L BA and 0.1 mg/L NAA).
Putrescine
(mg/L)
Number of shoots/explant
Shoot growth
(cm)
0 12.30 ± 0.8 1.20 ± 0.1
10 12.90 ± 1.3 1.32 ± 0.1
30 16.50 ± 1.7 1.45 ± 0.1
50 19.20 ± 1.6 1.79 ± 0.2
70 17.40 ± 1.2 1.51 ± 0.2
100 13.60 ± 1.1 1.32 ± 0.1
Each value is the mean ± standard error of 3 repeated experiments, with 50 explants used in each treatment.