The bacteria
were incubated at 37 ◦C with shaking at 175 rpm for 24 h. The
results are shown in Table 2. Accordingly, it is seen that the addition
of various inorganic nitrogen sources to the medium with
Fig. 1. Effects of soybean amount on milk-clotting activities in liquid-state fermentation
of Bacillus subtilis (natto) Takahashi in 24 h of cultivation.
or without the presence of soybean decreased the production of
milk-clotting activities.