The influenza reverse genetics system heavily depends on the cloning of influenza eight fragments into the plas-mids for influenza virus rescuing. Currently, the cloning processfor influenza genes into reverse genetics vector mainly requires
the restriction enzyme and ligation, and need considerable workfor successfully cloning the eight fragments of one influenza virusinto the reverse genetics vector. Therefore, speeding and simpli-fying the cloning process for influenza genes into reverse geneticssystem would benefit both influenza basic research and vaccinedevelopment