In order to identify appropriate eluting solvent mixture five different
acetonitrile–watermixtures, viz. 90:10, 70:30, 50:50, 30:70, 10:90 (v/v)
were used as eluting phases for the separation of the carbamate mixtures.
A mixed acetonitrile solution of all seven carbamates containing
10.0 mg/L of each was injected and choromatograms were developed
using above eluting phases with appropriate cleanup before each run.