2. Materials andmethods
2.1. Sample preparation
A total of sixty-nine shrimp that showed clinical signs of CMNV infections
including death shrimp, and shrimp ofwhitishmuscle necrosis,
abdominal muscle coloration, hepatopancreatic atrophy and necrosis
were collected from shrimp farms in four southern provinces of
Thailand. The samples were tested to verify that they had not been infected
by other virus and bacteria includingWSSV, IHHNV, TSV, YHV, infectious
mionecrosis virus (IMNV) and Vibrio parahaemolyticus. Shrimp
samples infected with other RNA viruses (i.e., TSV, YHV, or IMNV) were
also utilized to determine the cross reaction of other RNA virus with
CMNV.