Iodine test for detecting cellulase activity of bacte-rial cultures. (a) Pseudomonas sp. BSS2; (b)Bacillussp.
BSS3; and (c)A. xylosoxidans BSS4 with cellulolytic activity was selected using iodine test, which formed a bluish-black
complex with unused cellulose(CMC in the medium) leaving a sharp and distinct zone around cellulase producing
microbial colonies on BSM-agar plate with 0.5% CMC.
Clear zone mean no CMC, i.e., utilized by cellulase produced by the culture.