Avian leukosis viruses (ALVs) in poultry may induce a variety of deleterious effects including
tumors, increased mortalities, growth retardation and decrease in egg size and production that
led to considerable economic losses. The identification of avian leukosis viruses (ALVs) in
imported Marek’s disease (MD) vaccines has raised concern about transmission of these
retroviruses to vaccine recipients esp. poultry breeding stocks, so Egypt as one of importing
countries requires freedom of infection with ALVs in such vaccines. Subgroup specific RT-PCR
was undertaken on isolated RNA from 13 obtained commercial MD vaccines using six pairs of
primers that correspond to envelope glycoprotein gene (gp85) which determines possible
contamination with the six ALV subgroups: A, B, C, D, E, and J. The results indicated that RT-PCR
assay for ALV-gp85 subgroup-E was positive for eight out of thirteen (61.5%) tested MD vaccines,
while primers designed to detect subgroup A and J ALVs were positive for five out of thirteen
(38.5%) and two out of thirteen (7.7%) respectively among examined vaccines. No ALVs was
detected in 3/13 (23.07%) of commercially examined vaccines by using any of six primer pairs.
Finally, the using of RT-PCR assay provides us a new, sensitive approach for identifying ALVs as
a contaminant agent that will help greatly in applying this method for equipped labs as a quality
control measure for testing delivered MD vaccines before its administration in poultry breeding
stocks as well eradication programs through identifying infected birds.
Avian leukosis viruses (ALVs) in poultry may induce a variety of deleterious effects includingtumors, increased mortalities, growth retardation and decrease in egg size and production thatled to considerable economic losses. The identification of avian leukosis viruses (ALVs) inimported Marek’s disease (MD) vaccines has raised concern about transmission of theseretroviruses to vaccine recipients esp. poultry breeding stocks, so Egypt as one of importingcountries requires freedom of infection with ALVs in such vaccines. Subgroup specific RT-PCRwas undertaken on isolated RNA from 13 obtained commercial MD vaccines using six pairs ofprimers that correspond to envelope glycoprotein gene (gp85) which determines possiblecontamination with the six ALV subgroups: A, B, C, D, E, and J. The results indicated that RT-PCRassay for ALV-gp85 subgroup-E was positive for eight out of thirteen (61.5%) tested MD vaccines,while primers designed to detect subgroup A and J ALVs were positive for five out of thirteen(38.5%) and two out of thirteen (7.7%) respectively among examined vaccines. No ALVs wasdetected in 3/13 (23.07%) of commercially examined vaccines by using any of six primer pairs.Finally, the using of RT-PCR assay provides us a new, sensitive approach for identifying ALVs asa contaminant agent that will help greatly in applying this method for equipped labs as a qualitycontrol measure for testing delivered MD vaccines before its administration in poultry breedingสะท้อนเช่นโปรแกรมขจัดผ่านระบุนกติดเชื้อ
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