Materials
The pigment extracted from green-wheat-bran (Tang
et al. 2008): Added green-wheat-bran to 40% (v/v) acidified
alcohol (pH = 1.0), with the rate 1:15 (w/v), and
incubated the mixture at 60°C for 1 h to dissolve the
pigment, then centrifuged the extracted solution for 10
min (4 800 r/min), and collected the supernatant to vaporize
under 40°C by rotary evaporator, then freezedried
the concentrated pigment solution and got crimson
powder with yield of 15.1%