The PCR was performed on a
Dyand Thermal Cycler (MJ Research, Boston, MA, USA) under the
following conditions: one cycle each of 1min at 94 ◦C, one cycle of
40s at 92◦C, 40 s at 50 ◦C and 90 s at 75 ◦C followed by 30 cycles of
40s at 92◦C, 40 s at 50 ◦C, and 90 s at 75 ◦C. The final extension step
was at 75 ◦C for 5min. PCR products were quantified by 1% (w/v)
agarose gel electrophoresis with DNA Hyperladder IV (Bioline)