The next phase of our study was focused on monitoring of acrylamide 300 contents in
traditional soft breads from various Czech bakeries that employ practically the same dough
recipes and similar bread making technology, possibly only slightly differing in the way of
dough leavening. The results of crust analyses shown in Fig. 2 illustrate relatively small
variations of acrylamide contents among the crusts of traditional soft breads available at the
Czech market. The acrylamide content in 12 tested samples ranged from 7 to 22 μg.kg-1 with
a mean of 16 μg.kg-1. The calculated relative standard deviation for the data set was 30 %.
While in most cases, sourdough for leavening was prepared using a commercial mixture of
Lactobacilli as a starter together with commercial bakery yeast, the breads from bakeries no. 5
and 9, that contained the lowest acrylamide contents, were leavened using a natural rye
(home-made) sourdough obtained by spontaneous fermentation of rye flour. It contains
naturally originated microorganisms as Saccharomyces cerevisiae.