The second generation biomass to bio-ethanol production is of growing interest. Energy crop were
becoming important for second generation biomass to bio-ethanol production for their growth advantages.
Hybrid Pennisetum as a new hybrid energy crop was selected as a model to compare with corn
stalk. As pre-treatment methods, steam explosion and its combined action with dilute sulfuric acid,
bisulfite, and mixed dilute acid and bisulfite were selected. The enzymatic hydrolysis demonstrated that
the cellulose conversion is a strong function of the pre-treatment method applied, with corn stalk
providing slightly better results. With dilute acid steam explosion (DA-SE), conversions were 67.6% and
54.5% for corn stalk and pennisetum, respectively. This can be attributed to the higher Cr. I of pennisetum
(65.03%) than of corn stalk (54.05%). The cell lumen of pretreated pennisetum was smaller than for corn
stalk as shown in SEM photos, meaning there was a substantially higher enzyme accessible surface and
porosity in pennisetum, thus responsible for the higher cellulase adsorption of pretreated pennisetum.
DA-SE was the most effective pre-treatment method, but the inhibitors' concentration was higher than in
other methods. Combined dilute acid and bisulfite can moderately remove hemicelluloses and lignin. Cr. I
values, lignin content, accessible surface and porosity were supplied the energy crop evaluation standards
for bio-ethanol production.
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