Overall, EL resulted in a rela tively high cis -9 18:1 concentration and an increase in the 18:3n -3/18:2n-6 ratio , whereas combining EL and FO resulted in substantial increases in trans -FA, marginal enrichment in 20:5n -3 and 22:6n-3 and lower 16:0 concentration changes associated with a decrease in milk fat content