The residues of TM and its transformation product
MBC were determined in 1 ml of liquid medium
which was mixed with 4 ml of ethyl acetate (Merck,
Germany). The mixture was vortexed (30 s) and 3 ml
from the organic phase were removed and evaporated
to complete dryness in a rotary evaporator. The residue
was resuspended in 1 ml of a 50:50 (v:v) of acetonitrile:water which were used for HPLC analysis.