Pancreatic islets from adult female albino rats
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were isolated
according to the collagenase digestion technique.
Batches
of five islets were picked up using stereomicroscope and transferred
into small tubes each containing 1 ml KRH buffer supplemented
with 0.5% bovine serum albumin and glucose either
3 mmol/l (basal concentration) or 16.7 mmol/l (stimulatory
concentration) and the test agent under study was added.
The tubes were covered and incubated at 37 C in a shaking
water bath for 1 h with intermittent hand shaking every
15 min. At the end of incubation period the tubes were transferred
into ice bath and mixed with vortex mixer and then aliquots
of 0.5 ml were for insulin determination.
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