The obtained data
showed a negative effect by imidacloprid applied at the FR dosage for substrate-induced
respiration (SIR), the number of total bacteria, dehydrogenase (DHA), both phosphatases
(PHOS-H and PHOS-OH), and urease (URE) at the beginning of the experiment. In 10× FR
treated soil, decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over the
experimental period. Nitrifying and N2-fixing bacteria were the most sensitive to
imidacloprid. The concentration of NO3
− decreased in both imidacloprid-treated soils,
whereas the concentration of NH4
+ in soil with 10× FR was higher than in the control.
Analysis of the bacterial growth strategy revealed that imidacloprid affected the r- or K-type
bacterial classes as indicated also by the decreased eco-physiological (EP) index.
Imidacloprid affected the physiological state of culturable bacteria and caused a reduction
in the rate of colony formation as well as a prolonged time for growth. Principal component
analysis showed that imidacloprid application significantly shifted the measured parameters,
and the application of imidacloprid may pose a potential risk to the biochemical and
microbial activity of soils.
The obtained datashowed a negative effect by imidacloprid applied at the FR dosage for substrate-inducedrespiration (SIR), the number of total bacteria, dehydrogenase (DHA), both phosphatases(PHOS-H and PHOS-OH), and urease (URE) at the beginning of the experiment. In 10× FRtreated soil, decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over theexperimental period. Nitrifying and N2-fixing bacteria were the most sensitive toimidacloprid. The concentration of NO3− decreased in both imidacloprid-treated soils,whereas the concentration of NH4+ in soil with 10× FR was higher than in the control.Analysis of the bacterial growth strategy revealed that imidacloprid affected the r- or K-typebacterial classes as indicated also by the decreased eco-physiological (EP) index.Imidacloprid affected the physiological state of culturable bacteria and caused a reductionin the rate of colony formation as well as a prolonged time for growth. Principal componentanalysis showed that imidacloprid application significantly shifted the measured parameters,and the application of imidacloprid may pose a potential risk to the biochemical andmicrobial activity of soils.
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