20 g/l sucrose, vitamins glycine 2 g/l, pyridoxine 0.5 g/l, Nicotinic acid 0.5 g/l, Thiamine 0.1 g/l and Myo inositol at 0.1 g/l. The media also contained 0.8 g/l of activated charcoal (AC) to mimic the soil environment. It was supplemented with different concentrations of auxin as treatments for rooting; IBA with or without BAP as shown in (Table 2) and solidified with 4.5 g/l of agar. Each treatment was replicated five times and one explant was cultured in each culture bottle. The pH of the media was adjusted to 5.8 before addition of agar. The media were autoclaved at 121°C and 1.05kg/cm3 for 15 minutes. The cultures were incubated at 25°C ± 1°C and 16 and 8 hrs light and darkness respectively.