The HPLC system consisted of an Agilent 1200 series solvent
delivery system and UV-detector coupled with a C18 column
Ethanolic extracts of aloe gel
were eluted with a linear gradient from 100% solvent A (water)
and 0% solvent B (100% acetonitrile) to 84% solvent A and 16%
solvent B over 24 min. The gradient was then changed to 33%
solvent B over a 74 min period and then to 60% solvent B over a
100 min period,followed by 100% solvent B over a 120 min period
at a flow rate of 0.5 ml/min.The fractions were detected at
220 nm.