Background Phosphate buffered saline (PBS) is a balanced salt solution commonly used in the bio-laboratory. The essential function of a balanced salt solution is to maintain pH and osmotic balance as well as provide cells with water and essential inorganic ions. PBS is generally utilized to maintain cells for the short term in a viable condition while the cells are manipulated outside of their regular growth environment.One of the early formulas of PBS was developed by Renato Dulbecco, published in 19541 which are termed DPBS for Dulbecco’s phosphate buffered saline.