ammonium sulfate to the cell-free supernatant to obtain 60% saturation and stirred for 4 h at 4 C. After an additional centrifugation for 1 h at 12,000g at 4 C, the resulting pellet was re-suspended in 100 mL of
ammonium sulfate to the cell-free supernatant to obtain 60% saturation and stirred for 4 h at 4 C. After an additional centrifugation for 1 h at 12,000g at 4 C, the resulting pellet was re-suspended in 100 mL of