3.6.3 Soxhlet extraction Effect of solvent type 1) Weigh 0.5 g of Haematococcus pluvialis and place it in a thimble cartridge 2) Keep it in 500 ml round-bottom flask 3) Add 150 ml of acetone 4) Connect each part of the equipment 5) Place the equipment in the heater pocket (Electro mantle, USA) 6) Carry out the experiment at boiling temperature for 4 hour or until the debris becomes colorless 7) Change the solvent of the extraction in Step (3) to methanol, ethanol, and acetonitrile All experiments are carried out in triplicate.