For each sample contained in a zip lock bag, 4 pieces of cut spears (2 with apex and 2 with intermediate soft zone) were introduced, with an average weight of 8 g each sample and immersed in 8 ml of sterile brine composed by distilled water supplemented with 2% NaCl and 0.15% citric acid (pH 2.7)a and others that contain the central soft area