Explants were cultured on solidified MS media with agar (8 mg/l)
and its pH was adjusted to 5.7 before autoclaving at 121°C for 30
min. On cooling of the media, explants were cultured in Murashige
and Skoog (1962) media containing different concentrations of
auxins and cytokinins. One explant in each tube (15 × 2.5 cm)
containing 10 ml media was placed, vertically. Each treatment was
replicated three times and each replication has seven explants. The
tubes were covered with autoclaved poly praline sheets after
culturing, which were held in place with rubber band. The cultured
tubes were incubated for 16 h daily light of fluorescent, Philip white
tubes with intensified 1000 LUX, at 25 ± 1°C temperature