Sugar content determination was carried out by cold water extraction. To 3–5 g of dried coffee pulp 50 mL of demineralized water was added and the mixture shaken for 30 min. Afterward 2 mL of a 30% (w/w) ZnSO4 solution and 2 mL of a 15% (w/w) C6N6FeK4 solution were added. After shaking, the mixture was filtrated and the clear filtrate analyzed by HPLC as described above.