Saccharomyces cerevisiae is a non-pathogenic, highly
characterized eukaryotic microorganism [1]. It is widely used
in food and brewing industries, and various processes within
the production of renewable chemicals and pharmaceuticals
[2]. Also, significant progress towards improvement of producer yeasts is made via classical genetics, such as random
mutagenesis and evolutionary engineering [3].
In recent years, the availability of explosive whole-genome
information has thoroughly reshaped the strain reconstruction
studies for potential industrial applications [4]. Consequently,
the concept of minimum genome factories (MGFs) was proposed,which can be defined as recombinant strains whose
metabolism have been streamlined to the optimal minimal
subset applicable to specific utilities [5]. The construction of
MGF(s) generally involves multiple large-scale genomic