This actinobacterium also produces bio-surfactant, leading to the formation of true micelles, and enhancing the bioavailability of PAH for degradation. Further, the presence of yeast extract as a co-substrate in minimal medium significantly stimulated the degradation of 3–4 rings PAH.Our study shows that Streptomyces rochei is a good PAH degrader and the bioavailability of PAHs may be attributed to its biosurfactant production and emulsification ability. To the best of our knowledge, this is first report on degradation of PAH by Streptomyces rochei. This strain could be used for bioremediation of PAHs contaminated soils.