As it was already mentioned, the resolution of a 360 MHz NMR
spectrometer allowed the full proton NMR assignment of VLB in
1980 [56], almost 20 years after the first fragmentary 1H NMR
assignment of VLB was reported. In this study homonuclear decoupling
experiments were used in different media to assign all 1H
resonances and to determine their coupling patterns. The aim of
this study was to explore the conformation of VLB in a non-polar
solution (conformational questions will be further addressed in
Section 3.4).